P-91: Protective Effect of Black Grape Seed and Hawthorn Extracts on Cyclosporine A-Induced on In Vitro Fertilization Damge in Male Rat

Background: Infertility is a disease of the reproductive system that inhibits the ability of a couple to have a Baby. Cyclosporine A (CsA) is a powerful immunosuppressant drug most widely used in management of organ transplantation and autoimmune diseases. The drug has adverse side effects such, hirsutism, and gynecomastia. Hawthorn is one of the oldest herbal medicines that are widely used in medicine. Some crataegus constituents are predicted to be good antioxidants. Black grape extract contain a large amount of phenolic compounds in their skin, pulp and seeds. This study was conducted to investigate the effect of black grape seed and hawthorn extracts on CsA induced on in vitro fertilization (IVF) damge in male rat. Materials and Methods: In this study 32 adult male Rat, weighing 200 ± 20 g were used. The animals were divided into four groups (control, cyclosporine, cyclosporine+ Black grape seed extract, cyclosporine+ hawthorn extract). CsA were given at the dose of 30 mg/kg/day, hawthorn and black grape seed extracts at dose of 150 mg/ kg/day by gavage for 45 days. After 45 day the animals were sacrificed by dislocation of cervical vertebra, and fertility value from each group were studied Results: Cyclosporine caused decreased in sperm viability, motility, sperm count and level of serum testestrone .In our study, 2-cell embryos were stopped division and did not continue to blastocyct stage in cyclosporine group. Positive effect of Black grape seed and hawthorn extracts improvement of development embryo to blastocyct stage in treatment rats were considered. Conclusion: This investigation revealed that cyclosporine can decrease fertility in Rat but black grape seed and hawthorn extracts have improved embryo quality and increase zygotes, two celle embryo, and blactocycte.